The monitoring the growth rate of E.Coli bacteria is a useful indicator of the effect of glycotic enzyme mutation on the bacteria as the flow of intracellular metabolic components depends on the availability of carbon. Hence the change in carbon source can change the glyclyotic enzyme mutation up or down.
Explanation:
Continuous culture is a method that can be used by the researchers for determining whether mutation affects the growth rate of E.Colin-M bacteria
If the growth medium contains higher concentration of acetate,then the growth of the bacteria will be inhibited without inhibiting its central metabolism.
When E.Coli grows ,it secrets acetate. This mechanism is called overflow mechanism. Regulatory interactions mediated by acetyl-phosphate plays a major role in inhibiting growth by acetate. The uncoupling effect of organic acids or perturbation of the anion composition of the cell is a major reason for growth inhibition.
