Im sure its D
Empirical formula has the lowest number (simplified) version in an equation of molecules
Answer:
At pH 3.5:
Peptide A with pI 5.1 and peptide B with pI 8.5 will both bind to the anion-exchange column.
At pH 6.5:
Peptide A with pI 5.1 will bind to the cation-exchange column, while peptide B with pI 8.5 will bind to the anion-exchange column.
At pH 10.8:
Peptide A with pI 5.1 and peptide B with pI 8.5 will both bind to the cation-exchange column.
Explanation:
Ion-exchange column chromatography is used for separating proteins and other molecules in a solution based on differences in net charge. Negatively charged molecules bind to positively charged solid supports called the anion-exchange column, whereas, positively charged molecules bind to negatively charged solid supports known as cation-exchange column.
A peptide can acquire a net charge if the pH of the medium is either above or below its isoelectric point,pI. At pH above pI, the peptide acquires a net negative charge and can bind to a positive support. At pH above pI, the peptide acquires a net positive charge and can bind to a negative support. The greater the difference between pH and pI, the tighter the peptide will bind to the column.
At pH 3.5:
Peptide A with pI 5.1 and peptide B with pI 8.5 will both bind to the anion-exchange column.
At pH 6.5:
Peptide A with pI 5.1 will bind to the cation-exchange column, while peptide B with pI 8.5 will bind to the anion-exchange column.
At pH 10.8:
Peptide A with pI 5.1 and peptide B with pI 8.5 will both bind to the cation-exchange column.
A function of the number of electrons
Answer : A single carbon atom can form a maximum of <u>Four (4)</u> covalent bond(s).
Explanation : A covalent bond, also called a molecular bond, is a chemical bond that involves the sharing of electron pairs between atoms. These electron pairs are known as shared pairs or bonding pairs.
Formation of covalent bond depends on the number of valence electrons. Electronic configuration of Carbon (C) is : ![1s^{2},2s^{2},2p^{2}](https://tex.z-dn.net/?f=1s%5E%7B2%7D%2C2s%5E%7B2%7D%2C2p%5E%7B2%7D)
Valence electronic configuration of C is :![2s^{2},2p^{2}](https://tex.z-dn.net/?f=2s%5E%7B2%7D%2C2p%5E%7B2%7D)
Carbon have four valence electron that means Carbon can form maximum of four covalent bonds.
For example : In methane (CH4) Cabon can share four electrons with four Hydrogen atoms and can form four covalent bonds with 4 H.