Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Schizocoely is a process by which some animal embryos develop while splitting the mesodermal embryonic tissue. On the other hand, <span> enterocoelous was formed </span><span>in which the coelom forms from pouches "pinched" off of the digestive tract.</span>
Answer:Petroleum, also called crude oil, is a fossil fuel. Like coal and natural gas, petroleum was formed from the remains of ancient marine organisms, such as plants, algae, and bacteria. Over millions of years of intense heat and pressure, these organic remains (fossils) transformed into carbon-rich substances we rely on as raw materials for fuel and a wide variety of products.
Explanation:
Answer:
vizioner conter nu poltvinh
Explanation:
grandes comer ler coltrantes
To do the top of stored energy.
