Cation-exchange chromatography is used when the molecule of interest is positively charged, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. So, the amino acids with negative charge will elute the first. Glutamate, leucin, arginine is the order of elution because of their pI values ~3, ~6 ~10.
Answer:
1. 39 J; 2. 3.9 % usable; 3. 96.1 % unusable
Step-by-step explanation:
1. Available energy
Energy consumed = 1000 J
Less waste = - 177 J
Less respiration = <u>-784 J
</u>
Total energy lost = <u>-961 J
</u>
Available energy = 39 J
2. Percent of total energy usable
The chipmunk could use only 39 J of the original 1000 J.
% of energy usable = 39 J/1000 J × 100 % = 3.9 %
3. Percent of energy unusable
The chipmunk lost 961 J of the original 1000 J.
% of energy unusable = 961 J/1000 J × 100 % = 96.1 %
