Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
Answer:
C. Glucose and oxygen
Explanation:
Photosynthesis takes water, carbon dioxide, and light energy and turns it into cellular energy (glucose) and converts the carbon dioxide into oxygen. Plants are so vital to human life due to this process as they produce both the energy we need and air we breathe to survive.
During a myocardial infarction, proteins that are normally inside the cells leak into the plasma, due to loss of plasma membrane integrity.
<h3>Cardiac disorders and it's effects:</h3>
There are different types of cardiac or heart disorders that occurs due to different etiological origins.
Example of a cardiac disorder is the myocardial infarction.
Myocardial infarction is defined as the blockage of blood flow to the heart by the presence of blood clot.
This hinders the delivery of oxygen carried by the blood to tissue cells leading to apoptosis (which is a programmed cell death).
This will cause the plasma membrane of cells to lose its integrity leading to leaking of proteins into the plasma.
Learn more about myocardial infarction here:
brainly.com/question/1373449
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