Answer: C - life is information expressed
Explanation:
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
cell transport or membrane transport helps in the transport of solute molecules and ion across the plasma membrane and helps in maintaining homeostasis of these molecules in the biological system.
Explanation:
Homeostasis is very very important to maintain a steady body physiology and the maintenance of steady body physiology is highly significant because any type of disturbance in the homeostasis can leads various physiological abnormalities.
Various membrane transport process such as osmosis helps in the movement of water from high concentration region to the region of low water concentration until equilibrium is reached.Thus osmosis helps to maintain water homeostasis in our body.
By the same way glucose transporter maintain the homeostasis of glucose inside our body.
<span>Actually the developing offspring in the eight week of pregnancy is medically know as a fetus,Where the lungs of the baby are nearly developed, and baby continues to grow and mature, then reflexes of work starts woking slowly, after which baby prepares itself for delivery in next few days.</span>
Answer:
Dehydration Synthesis
The monomers combine with each other via covalent bonds to form larger molecules known as polymers. In doing so, monomers release water molecules as byproducts.