Answer:
You do not give warning about potential point lost
Explanation:
Teacher-class games are types of games instigated by the teacher to aid the learning process and improve communication among students. Points are given for positive behaviour and points are lost for negative behaviour. Part of the guideline for teacher-class games is that the students should not be warned about potential point lost or gain. The points should not be the motivating factor in the game.
These are the type of the story that put emphasis on the psychology aspects of the characters rather than the plot themselves. The characters here are exposed to dangers on a mental level. From the given choices, the best answer would be the mystery and suspense.
i would have to say the answer is B. No one wants to pay higher taxes just to recycle, so it would not be a incentive to do so. Hope this helps.
1. Samples of a microbial colony obtained from the natural environment are not pure culture. They are mixed cultures because the environment consists of an array of microorganisms and not one specific organism. A sample obtained from the natural environment such as water or soil may contain several species of microbes that need to be further isolated to obtain a pure culture.
2. Isolation streak plate technique involves the dilution of the sample several folds and then streaking a small amount using a sterile inoculation loop on an agar plate in a zig-zag manner. This is done to obtain a pure culture of bacteria from a mixed population by separating the microprocessor. When the sample is diluted into a small concentration and is streaked onto agar placed, widely spaced, discrete colonies of bacteria can be observed. These isolated colonies can be further cultured to obtain pure cultures by picking and re-streaking them on fresh agar plates.
3. A microbial colony is considered a pure culture because it consists of single species. A single species of bacteria cluster and form an isolated colony on an agar plate which can be further plated on fresh medium.
3-1 - If your agar plate shows discrete uncluttered colonies then you have obtained an isolated colony. The following picture might help you to understand.
3-2. If you subculture isolated microbes into a sterile nutrient broth without any contamination, it will still be considered pure culture because it is single isolated species in a sterile aseptic environment of the nutrient broth.
3.3. A pure culture can become a mixed culture due to contamination. This could be caused by airborne bacteria if your conditions are not aseptic. Contamination is possible if the work area is not clean and sterile or if the glassware is not sterile. The contamination can transform your pure culture into a mixed culture and is hence problematic. So, it is very important to maintain a sterile and aseptic environment, glassware, and instruments.
Learn more about subcultured at
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Answer:
pretty sure the US bought alaska for dirt cheap, even by 19th century standards.
