Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Answer:
B
Explanation:
As the distance between the planets and the sun increases, the period of revolution increases as well. The period of revolution is how long it takes for a planet to revolve around the sun. So, because the planets farther from the sun have a higher period of revolution in earth years, this also means they have longer actual years, which means the answer is B.
Number of proton present in the nucleus determines the atomic number of an element. It determines <span>chemical properties, which is why all atoms with proton count (atomic number) 6 are carbon</span>
This is because amino group of p-aminobenzoic acid is an aniline and is less electrophilic than an alkyl amine.
<h3>What is an Aniline?</h3>
This is an aromatic amine which consists of a phenyl group attached to an amino group.
The amino group of p-aminobenzoic acid being an aniline makes it less electrophilic which is why an alkyl amine participates in the reaction instead.
Read more about Aniline here brainly.com/question/9982058
The waters of the Dead Sea are extremely saline, and, generally, the concentration of salt increases toward the lake's bottom. ... The deep water was saturated with sodium chloride , which precipitated to the bottom.
