Through the urethra which is in a mans' penis or a woman's vagina.
We all know that heat is energy, and if too much energy is applied to anything, it is denatured. pH is a denaturing agent since it changes the protein, essentially by using Hydrogen (part of the bonds) as a counteract. Amphiphilic detergents are not beneficial for proteins because they’re responsible for breaking covalent bonds. Mercaptoethanol, as the name says, has ethanol, which reduces proteins into nonexistence.
It would be 50 degrees Fahrenheit
Both the temperature and the pressure increase.
