1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
myrzilka [38]
3 years ago
11

Recombinant DNA technology involves which sequence of steps?

Biology
2 answers:
MrRissso [65]3 years ago
7 0
In standard cloning protocols, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host organism and cloning vector, (2) Preparation of vector DNA, (3) Preparation of DNA to be cloned, (4) Creation of recombinant DNA, (5) Introduction of recombinant DNA into the host organism, (6) Selection
liraira [26]3 years ago
7 0

Answer:

B

Explanation:

Processes of recombinant DNA technology involve isolation of DNA of the desired DNA fragment. In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macromolecules. The DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides, and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus). The RNA can be removed by treatment with ribonuclease whereas proteins can be removed by treatment with protease.

You might be interested in
A client with suspected myasthenia gravis is to undergo a test with edrophonium. the client asks if edrophonium can be used to t
olga nikolaevna [1]

The correct answer is: the short half-life of edrophonium makes it impractical for long-term.

Edrophonium is used for the diagnostic of myasthenia gravis. In patient with myasthenia gravis, the body produces autoantibodies which inhibit nicotinic acetylcholine receptors in the neuromuscular junction. Edrophonium, on the other hand, is acetylcholinesterase inhibitor which blocks the effect of acetylcholinesterase enzymes (AcH stays in synaptic cleft).

Substitution for the edrophonium is pyridostigmine which is also acetylcholinesterase inhibitor but with long-term maintenance.


6 0
3 years ago
How much work is done when you use 425 N of force to move a 15 kg box 2.8 m?
kherson [118]

Answer:

1190 J

Explanation:

3 0
3 years ago
When oxygen is not available, lactic acidosis fermentation most likely occurs
guajiro [1.7K]
B muscle cells because lactic acid is what makes your muscles sore when there isn’t enough oxygen
7 0
3 years ago
Read 2 more answers
What type of microscope would be best for viewing the samples you created in real life?
makkiz [27]

Answer:

The type of microscope depends upon the type of sample created.

For observing a sample just to check out its presence of any substance, you can use a simple microscope, and to observe a sample by its morphological features with varying objective lens, you can use compound microscope. To study deep details of the sample, a microscope of high magnifying power like electron microscope can be used.  

6 0
3 years ago
Blank control all function of the body
solong [7]
System purifies the body by the elimination of waste products. This system enables breathing, supplies the body with oxygen, and eliminates carbon dioxide as a waste product. This system serves as a productive covering for the body and helps in temperature regulation.
6 0
3 years ago
Read 2 more answers
Other questions:
  • Any help??? It would be appreciated!
    6·1 answer
  • Mechanical weathering ____.
    10·2 answers
  • What does Phosphorus cycle between
    8·1 answer
  • What scientific practice is Rhonda performing on what effect the air pressure at high altitude has on a volume of gas in baloons
    5·1 answer
  • Which of the following can be categorized as prokaryotic
    14·1 answer
  • A newly discovered gene has two aleles a and a scientists hypothesize that the alleles show incomplete dominance
    7·1 answer
  • What do we us everyday that harms us?
    15·2 answers
  • mitosis A division happens in the next step. Which describes the cells after the next step is complete? A. The cells will have n
    13·1 answer
  • 1. List four sensations detected by the tactile receptors in the skin in the spaces below:
    11·1 answer
  • All sources of pollution come from human activities. please select the best answer from the choices provided t f
    15·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!