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myrzilka [38]
3 years ago
11

Recombinant DNA technology involves which sequence of steps?

Biology
2 answers:
MrRissso [65]3 years ago
7 0
In standard cloning protocols, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host organism and cloning vector, (2) Preparation of vector DNA, (3) Preparation of DNA to be cloned, (4) Creation of recombinant DNA, (5) Introduction of recombinant DNA into the host organism, (6) Selection
liraira [26]3 years ago
7 0

Answer:

B

Explanation:

Processes of recombinant DNA technology involve isolation of DNA of the desired DNA fragment. In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macromolecules. The DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides, and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus). The RNA can be removed by treatment with ribonuclease whereas proteins can be removed by treatment with protease.

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Why do the cells need to divide
sergey [27]

Answer:

Cells divide so living things can grow. When organisms grow, it isn't because cells are getting larger. Organisms grow because cells are dividing to produce more and more cells.

5 0
3 years ago
Which molecule would be the most affected by limited nitrogen?
stealth61 [152]

Answer:

The molecule that would be affected by 'limited nitrogen' is the myofibrillar proteins in muscle. Explanation: The myofibrils of the muscle have a number of protein molecules.

5 0
3 years ago
HELP! This is the amount of oxygen present at a specific time that can be used in aerobic cellular respiration.
skad [1K]

Available oxygen

Available oxygen is the amount of oxygen present at a specific time that can be used in aerobic cellular respiration.

Aerobic cellular respiration is a metabolic process that occurs within the cells of organisms. In this process, oxygen is used in the mitochondria to chemically convert organic molecules such as glucose into adenosine triphosphate (ATP), with the release of water and carbon dioxide as waste products. Aerobic cellular respiration results in a larger amount of energy (ATP) which is used by the cell to perform its activities.

5 0
3 years ago
Read 2 more answers
Name two types of evidence scientists use to support the theory of evolution. *Hint* The human hand is very similar to a Dolphin
Alex73 [517]

Answer:

Hello! There are numerous things that support Darwin's theory of evolution, but the most credible ones include:

*Homologous structures - structures that are similar in structure but different in function (e.g. wing of a bat and arm of a human).

*Embryology - the study of embryos

*Continental drift - some fossils of organisms that were adapted to a different environment are often found in different climates/areas they don't seem to have been adapted to.

*DNA - similarities in the genomes of certain animals are often used to compare amino acid sequences and protein production.

I hope I helped!

There are a bunch more, but I decided to leave a select few for you to write about.

Feel free to leave a comment down below if you need more assistance. :)

4 0
3 years ago
How many kingdoms are there in the domain Eukarya​
Marina CMI [18]

Answer:

five kingdoms

Explanation:

members of the domain eukaryotes have member-bound organelles including a nucleus containing genetic material and are represented by five kingdoms.

4 0
3 years ago
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