Question

To run a spectrophotometry experiment, begin by _______ the spectrophotometer and preparing the samples. Be sure to select the correct ________, then run a measurement on the _______ solution. Follow up by running measurements on _______ solutions. Once data is collected, turn off the instrument, clean the area, and discard the samples.
1.
a) cleaning
b) warming up
c) unplugging
2.
a) wavelength
b) transmittance
c) absorbance
3.
a) blank
b) aqueous
c) sample
4.
a) blank
b) aqueous
c) sample

Answer 1

Answer:

b) warming up a) wavelength a) blank c) sample

Explanation:

To run a spectrophotometry experiment, begin by warming up the spectrophotometer and preparing the samples. It is important that the equipment is warmed up for at least 30 minutes before starting the measurements.

Be sure to select the correct wavelength, then run a measurement on the blank solution. The selected wavelength depends on the analyte of interest. The black solution contains the same matrix but it doesn´t contain the analyte.

Follow up by running measurements on sample solutions. Once data is collected, turn off the instrument, clean the area, and discard the samples. The samples are those of unknown concentration that we want to determine.