Is said to be denatured.
Denaturation occurs when the environment around has a extremely high temperature of extreme pH values comparing to the optimum values of the enzyme. Each enzyme has their own optimum temperature and pH.
In the case, the lost its three-dimensional structure and can no longer function because the shape of the active site is altered and thus it can no longer bind into substrates.
Answer:
I'm pretty sure it is metamorphosis.
10 ng is the minimum amount of DNA to visualize it on agarose gel. The amount of DNA to load per well is variable. The least amount of DNA that can be detected with ethidum bromide is 10 ng.