An isolated colony on a selective medium is not considered a pure culture because inhibited organisms might be masked by the colonies. This statement is true.
On the basis of their constitution or usage, culture media can be classified into various groups; these include defined, complex, selective, as well as enrichment medium.
A selective medium consists of dyes or noxious substances/chemicals which inhibit the growth of specific microorganisms but promote the growth of other microbes.
A pure culture is a laboratory culture which contains a single species of microorganism. It is free from other microorganisms.
Since selective media affects the growth of distinct organisms differently, therefore, an isolated colony formed in such type of medium is not considered a pure culture.
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I think it will be RNA for both questions
Answer: Replication occurs in three major steps: the opening of the double helix and separation of the DNA strands, the priming of the template strand, and the assembly of the new DNA segment. During separation, the two strands of the DNA double helix uncoil at a specific location called the origin. Several enzymes and proteins then work together to prepare, or prime, the strands for duplication. Finally, a special enzyme called DNA polymerase organizes the assembly of the new DNA strands. The following description of this three-stage process applies generally to all cells, but specific variations within the process may occur depending on organism and cell type.
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