The heart is a part of the Circulatory system.
We use the gas law named Charle's law for the calculation of the second temperature. The law states that,
V₁T₂ = V₂T₁
Substituting the known values,
(0.456 L)(65 + 273.15) = (3.4 L)(T₁)
T₁ = 45.33 K
An educated guess is called estimation
