Which methods could be used to dilute a solution of sodium chloride

Chemistry

1 answer:

Brrunno [24]2 years ago

8 0

Answer:

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3 years ago

The Michaelis constant for pancreatic lipase is 5 mM. At 60 C, lipase is subject to deactivation with a half-life of 8 min. Fat

Dmitriy789 [7]

Explanation:

According to the given data, we will calculate the following.

Half life of lipase $t_{1/2}$ = 8 min x 60 s/min

= 480 s

Rate constant for first order reaction is as follows.

$k_{d} = \frac{0.6932}{480}$

= $1.44 \times 10^{-3}s^{-1}
$

Initial fat concentration $S_{o}$ = 45 $mol/m^{3}$

= 45 mmol/L

Rate of hydrolysis $V_m_{o}$ = 0.07 mmol/L/s

Conversion X = 0.80

Final concentration (S) = $S_{o} (1 - X)$

= 45 (1 - 0.80)

= 9 $mol/m^{3}$

or, = 9 mmol/L

It is given that $K_{m}$ = 5mmol/L

Therefore, time taken will be calculated as follows.

t = $-\frac{1}{K_{d}}ln[1 - \frac{K_{d}}{V}{K_{M} ln (\frac{S_{o}}{S}) + (S_{o} - S)]$

Now, putting the given values into the above formula as follows.

t = $-\frac{1}{K_{d}}ln[1 - \frac{K_{d}}{V}{K_{M} ln (\frac{S_{o}}{S}) + (S_{o} - S)]$

= $-\frac{1}{1.44 \times 10^{-3}s^{-1}}ln[1 - \frac{1.44 \times 10^{-3}s^{-1}}{0.07 mmol/L/s
}{K_{M} ln (\frac{45 mmol/L
}{9 mmol/L
}) + (45 mmol/L - 9 mmol/L
)]$