Enzymes are important catalysts that speed up the rate to chemical reactions in cells. How do they accomplish this?
1 answer:
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Answer:
TCA cycle in particular is inhibited by fluoroacetate. Fluoroacetate is a compound the binds to the Coenzyme A of the Krebs cycle to form Floroacetyl CoA which in turn forms fluorocitrate instead of forming citrate. This fluorocitrate is not able to bind to aconitase enzyme preventing the conversion in to isocitrate, thereby halting the Krebs cycle.
a) The inhibitor molecule here is Fluorocitrate.
t a Coa-sa Co ASH Coenzyme A Fro Nat Fluoroacetate Fluoroacetyl COA Citrate Hooc-ch-F Hooc-c-one Hooc-ch, Fluoro citrate
b) see attachment below
c) The fluoroacetate along with Coenzyme A forms Fluoroacetyl CoA which in turn forms Fluorocitrate in place of citrate formation. Fluorocitrate does not bind to Aconitase hance isocitrate is not formed and the Krebs cycle in halted.
See attached file for the continuation of c.
The above mechanism shows the inhibition of aconitase, where the presence of fluorine causes resonance to occur after the flip step preventing the Ser 642 moiety of Aconitase from binding and no isocitrate conversion.
d) As compared to the normal tissues the, due to the inhibition of aconitase enzyme, the citrate isocitrate conversion will proceed at a very slow rate thereby halting TCA cycle and the concentrations of the further produced components:
Citrate concentration increased
Isocitrate concentration reduced
Alpha - ketoglutarate concentratioin reduced
Succinyl CoA concentration reduced
Succinate concentration reduced
Fumarate concentration reduced
Malate concentration reduced
Oxaloacetate concentration reduced
