Question

The primers you used in lab amplified 145 base pairs that were adjacent, but not included in the repeat. If you used a different primer that only amplified 130 base pairs adjacent to a 14 base pair repeat, and your PCR product size was 172, how many repeats were in your product?
6
5
1
4
3
2

Answer 1

Answer:

3 repeats

Explanation:

PCR technique used to make multiple DNA copies from a segment or small sequence of DNA that is called primer for identification or detection of the pathogen.

given:

Primer amplified base pairs - 130  that are adjacent to - 14 base pair repeats.

PCR product size was 172

So, the extra nucleotide that we get would be = product size - primer amplified

172- 130 = 42 bp

Extra repeats you got = 42/14= 3 repeats