Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Chemoreceptor is a specialized sensory receptor cell, which responds to a chemical substance and generates a biological signal.
The type of chemoreceptor responsible for our sense of smell is called olfactory receptor. It is also known as odorant receptor, these are found in a small patch of tissue high inside the nose. These cells directly connected to the brain.
Protein synthesis occurs in the cytoplasm, specifically in the ribosomes. Proteins that are meant to be excreted, such as in the pancreas, are produced in the rough endoplasmic reticulum which is a membrane studded with ribosomes. In organs that primarily function to produce and secrete proteins, there will be a larger rough endoplasmic reticulum with more ribosomes.
I’m not sure but it’s definitely a stimulus and I think if it wants specific terms it is a Conditioned Stimulus
Answer: Metalloid and non-metals not only form covalent bonds by sharing, but can form ionic bonds either by losing or gaining electrons
Hope this helps
Explanation:
