Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
a solution of each sodium salt at the same concentration
Explanation:
If a scientist wishes to study the electrical potential that develops between ice and the sodium salts solution of the ions (F-, Cl-, Br-, or I-) in order to determine which ion produced the greatest electrical potential; he must compare the electrical potential that develops between ice and a solution of each sodium salt at the same concentration.
This means that conclusions can not be reached unless the sodium salt of each ion at the same concentration is compared so as to ensure consistence in the results of the study.
Parietal cells produce gastric acid (hydrochloric acid) in response to histamine (via H2 receptors), acetylcholine (M3 receptors) and gastrin (gastrin receptors). Parietal cells contain an extensive secretory network (called canaliculi) from which the HCl is secreted by active transport into the stomach.
Interphase in the cell cycle encompasses the G1, S, and G2 phases, as it shows the period of growth and DNA replication that a cell must go through to prepare for mitosis. Cell division, which occurs during the M phase, is the only portion of the cell cycle that is not included in interphase.
The pattern of change in CO₂ concentration is new plant growth in the spring increases photosynthesis rates, which lowers CO2 concentrations; this decline continues until autumn, when leaves fall and decompose, raising CO2 concentrations.
The level of carbon dioxide within the surroundings rises and falls every 12 months as plant life, thru photosynthesis and respiration, absorbs the gas in the spring and summer season, and launches it in fall and wintry weather. Now the range of that cycle is expanding as extra carbon dioxide is emitted from burning fossil fuels and different human activities.
In spring, flora in the Northern Hemisphere starts to develop and absorb CO2 from the atmosphere, so atmospheric CO2 attention decreases as the road dives down. In fall, flora starts to decay and release their CO2 again into the ecosystem, so atmospheric CO2 attention increases the line shoots up. plants are accumulating carbon within the spring and summer while they are lively, and they're liberating carbon again to the air in the fall and winter.
Learn more about patterns that occur here:-https://brainly.ph/question/6286565
#SPJ4
