Salt is better correct answer
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
1: Population; 2: Biome;3: Ecosystem
Explanation:
Population refers to the number of people in a state. Biome is a large area characterised by it's vegetation, soil,climate, and wildlife. Ecosystem is a geographic area where plants, animals and other organisms exists
I would also say that the answer is C
Viral<span> particles, also known as virions, consist of two or three parts: (i) the </span>genetic<span> material made from either DNA or RNA, long molecules that carry </span>genetic information; (ii) a protein coat, called the capsid, which surrounds and protects thegenetic<span> material</span>
