Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
Plants compete with eachother for necessary resources. They compete for nutrients, light, water, space, and pollinators.
Explanation:
hope this helps :)
Answer:
The members of the swine species <u>always have the same number and kinds of chromosomes</u>.
Answer:
Comparative embryology is one of the main lines of evidence in support of evolution. In comparative embryology, the anatomy of embryos from different species are compared through the embryos development. Similarities between different species indicate that we all came from a common ancestor.
Explanation:
