Roche/454 pyrosequencing , useful for high throughput sequencing techniques. This method utilizes pyrosequncing which allows for sequencing as the sequence read out can be achieved at the same time as the sequence is extended.
High- throughput screening is the use of automated equipment to rapidly test thousand to millions of samples for the biological activities at the model organism, cellular, pathway or molecular level.Many DNA samples can be sequenced simultaneously.
Sanger DNA sequencing also known as chain termination. This method is used only for the single DNA fragment at a time. Amplicon sequencing with NGS is efficient and cost effective . It is required a DNA polymerase , nucleotide , dideoxynucleotides and buffer.
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Answer: Cushing Syndrome
Explanation:
Cushing Syndrome occurs when the body is exposed to high level of cortisol for a long time either externally (taken into the body from outside ) or internally (secretion in excess of the hormone called cortisol by the body) . It can also be called hypercortisolism .
The symptoms of Cushin Syndrome may vary depending on the severity of the excess cortisol secretion. Females may have irregular menstrual period due to this condition.
Asthma and arthritis patients who take corticosteroids and experiences the mentioned symptoms should seek medical attention for diagnosis.
D. population and nutrients
Answer:
Immediately, the pathogen has been recognized:
Macrophages acts as the first line of defence by engulfing pathogens identified by antigens which will now present the antibody shape to a helper T cell.
The Helper T cells produce a signal to plasma and Memory B cells to yield antibodies that attach to the antigens. The cytotoxic cells that leads to cell death are activated by the helper T cells.
Antibodies helps to immobilize pathogen for macrophage to feed on.
if the pathogen comes back a 2nd time the memory cells helps in quick and efficient recovery by producing the specific B and T cells for the antigen.
