1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Tpy6a [65]
2 years ago
7

In the ELISA procedure, first a protein sample is immobilized to a plastic well. Then, the antibody to the protein of interest i

s added, incubated, and the excess is washed out. Next, the detection antibody (which binds to the primary antibody) is added, incubated, and the excess is washed out. The last step is to add the detection dye (which binds to the detection antibody) to the wells and allow it to incubate. What do you predict the result would look like if you added the detection antibody first, then the antibody to the protein of interest
Biology
1 answer:
mario62 [17]2 years ago
8 0

Answer:

In this ELISA procedure, a sample of protein is immobilized in the plastic well and then antibody for this protein added and incubated, and cleared out. In the given setting detection, the antibody is conjugated with the protein of interest and if the substrate molecule added to the reaction it will produce a colored product by reacting with the enzyme.

No reaction will take place in the well in case of not adding secondary antibody or detection antibody to the reaction and no colored product will be received.

You might be interested in
Which of the following is a reason why sticky end producing restriction enzymes are preferred compared to blunt end ones?
anyanavicka [17]

Answer:

D) Because sticky ends can be temporarily held together by hydrogen bonding between the two strands.

Explanation:

Restriction enzymes cut the DNA at specific restriction sites and by the mechanism of action they can form two types of ends:

  • sticky ends-single-stranded overhangs are formed
  • blunt ends-without overhangs.

The main advantage of sticky ends (their overhangs) is that they can complementary bind to another overhand formed by the same restriction enzyme. So, for example in cloning, if the DNA of interest and plasmid vector are cut with the same restriction enzyme, that  forms sticky ends, fragment of DNA will fit into a bacterial plasmid in one direction.

On the other hand, blunt ends can be inserted into vector in both directions: head-to-tail or tail-to-head.

6 0
2 years ago
In which environment would mineral formation caused by high pressures and high temperatures most likely occur?
fenix001 [56]
Lava flowing because it would put a lot of weight(pressure) and high temperatures
5 0
3 years ago
What features or characteristics does a real statement have?
Inga [223]
In order to be effective, the following characteristics of mission statement need to be present in an organization. 1. It should be feasible A mission should always aim high but it should not be an impossible statement. It should be realistic and achievable its followers must find it to be credible.
3 0
3 years ago
Why is setting goals an important part of continued fitness?
vazorg [7]
Without goals motivation is almost impossible
4 0
3 years ago
Which mutation occurs when one nucleotide base is replaced with another base
svetlana [45]

The correct answer is a substitution mutation.  

A mutation, which substitute’s one base for another, that is, a change in a solitary chemical letter like switching an A to a G is known as a substitution mutation. This kind of substitution can modify a codon to something, which encrypts a distinct amino acid and leads to a small modification in the generated protein.  


6 0
3 years ago
Read 2 more answers
Other questions:
  • The process that releases energy from food in the presence of oxygen is ?
    12·1 answer
  • Which of the structures shown above contains a nucleolus?
    6·1 answer
  • What is the definition of andesitic composition ?
    9·2 answers
  • The body cavity of an annelid is a true coelom.<br> a. True<br> b. False
    9·1 answer
  • Which physical property is best measured using only a balance?
    12·1 answer
  • What is the ratio of land to water on earth
    12·1 answer
  • Metabolism is a term used to describe all the chemical processes that go on
    10·1 answer
  • List these biomes in order from most biodiversity to least: grasslands, dessert, taiga, rainforest
    7·1 answer
  • What happens to energy that is released from food, but not captured and store into<br> ATP?
    6·1 answer
  • What percentage of the moon as seen from Earth is illuminated during the third quarter phase?
    5·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!