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Tpy6a [65]
2 years ago
7

In the ELISA procedure, first a protein sample is immobilized to a plastic well. Then, the antibody to the protein of interest i

s added, incubated, and the excess is washed out. Next, the detection antibody (which binds to the primary antibody) is added, incubated, and the excess is washed out. The last step is to add the detection dye (which binds to the detection antibody) to the wells and allow it to incubate. What do you predict the result would look like if you added the detection antibody first, then the antibody to the protein of interest
Biology
1 answer:
mario62 [17]2 years ago
8 0

Answer:

In this ELISA procedure, a sample of protein is immobilized in the plastic well and then antibody for this protein added and incubated, and cleared out. In the given setting detection, the antibody is conjugated with the protein of interest and if the substrate molecule added to the reaction it will produce a colored product by reacting with the enzyme.

No reaction will take place in the well in case of not adding secondary antibody or detection antibody to the reaction and no colored product will be received.

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Answer:

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Cardiovascular effects of the sympathetic division include all of the following EXCEPT dilation of the blood vessels serving the skin and digestive viscera.

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Answer:

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The dependent variable is the variable that responds to changes of the independent variable. In other words, the dependent variable is the outcome of manipulating the independent variable. Hence, the dependent variable is dependent on the independent variable. For Rachel's experiment, the PLANT GROWTH is the dependent variable because it is what responds to changes in amount of light (independent variable).

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