Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
<h3>In taxonomic, the organism is classified based on some similarities. ... But the organism with the same order should have the same phylum and class too since order is located below the phylum. That means the organism with the same order should have more similarities than the organism with the same phylum.</h3>
From the options that factor that does not effect the rate of enzyme reaction is : ( D ) Amount of product.
Enzymes are substances found in organisms that catalyzes the rate of chemical reaction occurring in an organism. during the chemical reaction the enzyme remains intact and is not utilized during the process.
Some of the process that may Inhibit the performance of an enzyme(s) includes ;
- pH ,
- The substrate concentration,
- Temperature and
- The concentration of the enzyme.
The amount of the product does not affect the performance rate of the enzyme because enzymes functions in chemical reactions and not physical reactions
Hence we can conclude that the factor that does not effect the rate of enzyme reaction is the Amount of product
Learn more : brainly.com/question/13981863
Answer:
I think it's A tell me if it's wrong
hope it helps
have a good day :)
Explanation:
Answer:
Damage to the heart muscle
