Answer: Nucleotides are the monomers used for DNA synthesis. The mix also contains a template, DNA taq polymerase, buffer, reverse and foward primer and magnesium ions.
Explanation:
A PCR master mix is a premixed solution that has all of the components for a Polymerase chain reaction (PCR). This reaction is a laboratory technique that amplifies small fragments of DNA into millions of copies.
The master mix used for that contains dNTPs (nucleotides). In the DNA there are four types of nucleotides that are differentiated by the nitrogen base they have: adenine (A), guanine (G), cytosine (C) and thymine (T).
<u>Since nucleotides are the monomers that make DNA, they are found in the mix because they are the material for DNA synthesis.</u>
The reaction mixture also requires;
- DNA template, the sequence of DNA to be amplified
- DNA taq polymerase, a heat resistant enzyme that assembles nucleotides into a new DNA
- Salt buffer, for an optimum ionic environment and pH
- Oligonucleotide primers (reverse and foward), pieces of DNA complementary to the template. Each hybridizes with one of the DNA chains.
- Magnesium ions, a catalyst required by DNA polymerase to work
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Answer: A B C
Explanation:
The synthesis of ATPS takes place in the mitochondrial and chloro plasts of cells.It is an exergonic reaction that involves the use of energy released from influx of protons to for the catalysis of in organic phosphate and ADP by enzyme ATP Synthase in the stroma and matrix of chloroplasts and mitochondrial respectively.
The 3 bonds that connected phosphate ions to ADP to form ATP must be broken down(hydrolysis) with water to release the energy for biochemical activities of the cells.
The first bond hydrolysis releases first phosphate and ATP changed to ATP,
The second bond releases breakage changed the ADP to AMP with the release of more energy.
It produces new, organic, rich soil, that can contribute to agriculture and farming, as well as the decomposers that live in them. It is very good to compost.
