Question

the green structures are Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin. Through the microscope, the green structures are endospores. capsules. cell walls. flagella. The answer cannot be determined.

Answer 1

Answer:

The correct answer is:

endospores

Explanation:

endospores are spores formed by certain genera of bacteria eg, Bacillus. Spores generally are formed by the vegetative (growing) part of bacteria cells in response to adverse or unfavorable conditions. Spores are inactive and dehydrated and can stay dormant for a very long time, only germinating when the conditions are right. The outer covering of spores are made of tough protein coats, hence they do not take up conventional stains, but special staining methods like Malachite green can be used, and since the method makes use of heating, the primary stain (Malachite green) is forced into the tough coat of the spores (stains green), and resists the decolorization step, while the vegetative cells are easily decolorized, and take up the counterstain (Safranin), which stains the parent cells red.