1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Tomtit [17]
3 years ago
8

You are trying to program E. coli bacteria to generate N-acylethanolamide, lipids that have been found to help suppress appetite

. In order to do this, you need to transfer the gene for the enzyme N-acyltransferase from the plant Arabidopsis Thaliana into the E.coli bacteria.
The transgene, which codes for ***********, will also need to have a**********
for RNA polymerase and a ********** that would fluoresce to let you know the insertion was successful. You would also need a********** , a small circular piece of bacterial DNA.
Then you would hunt for a *********** that would cut the plasmid once and also cut the transgene out from its host.

FILL IN THE **********
Biology
1 answer:
gulaghasi [49]3 years ago
6 0

Answer:

The transgene, which codes for N-acylethanolamide, will also need to have a transcription initiation site for RNA polymerase and a selectable marker (reporter gene) that would fluoresce to let you know the insertion was successful. You would also need a plasmid, a small circular piece of bacterial DNA. Then you would hunt for restriction enzymes that would cut the plasmid once and also cut the transgene out from its host.

Explanation:

A transgene can be defiend as a gene (in this case, the N-acylethanolamide gene) which has been transferred by using genetic engineering techniques from one organism to another. The RNA polymerase is an enzyme required to copy a DNA sequence (e.g., a gene) into an RNA sequence, during transcription. RNA polymerases bind to specific DNA sequences called transcription initiation sites on the DNA template, and this binding triggers the initiation of the transcription. A plasmid is a small circular DNA molecule separated from chromosomal DNA which is capable of replicating independently of the chromosomal DNA. Plasmids are widely used in genetic engineering to clone/transfer/manipulate transgenes. During transgenesis, the plasmid is cut with a specific restriction enzyme in order to ligate the transgene of interest into the plasmid. Finally, the green fluorescent protein (GFP) gene is a selectable marker (i.e., a reporter gene) that indicates which cells were successfully modified because its expression can be detected without any substrates.

You might be interested in
Skin has epithelial cells that resist stretching and twisting because they are held together by what
KatRina [158]

The epithelial cells resist stretching and twisting in the skin because if desmosomes that holds them together. The desmosomes are the one responsible for the cell to cell adhesions, making them be tight or held together as this is its structure.

7 0
3 years ago
Why does the enzyme pepsin (present in the stomach) denature in the intestine?
Art [367]
I think the correct answer from the choices listed above is the second option. The enzyme pepsin that is present in the stomach denature in the intestine due to the alkaline environment of the intestine. This is because pepsin has an optimum pH that is very low which is ideal for the acidic conditions of the stomach. 
4 0
3 years ago
Read 2 more answers
Can somebody help me with thispls!!
babymother [125]
Are their options? I think I need more info
8 0
2 years ago
Which explanation is most likely the reason for the evolution of a larger brain in humans?
Alinara [238K]

Answer:

The correct statement is A larger brain allows humans to solve complex problems.

Explanation:

From the ancient time, the human brain has size increased three times. The most probable reason for the evolution of brains is that larger brain allows humans to handle complex problems more easily.

complex and large brains help humans to understand and store a different type of knowledge and information and aids in interacting with each other.  

The current time human has a larger and complex brain in any living primates.

Thus, the correct statement is A larger brain allows humans to solve complex problems.

6 0
3 years ago
Please answer!! I will give you brainliest!!!!!!
Serga [27]

warm front

// answer was short so added this text //

5 0
3 years ago
Other questions:
  • The third stage of cellular respiration is (2 points)
    14·2 answers
  • What precautions should one use when working with phenacetin?
    10·1 answer
  • Cells are placed in a solution that causes them to absorb liquid until they burst. When more water is removed from the solution,
    8·1 answer
  • Please help me answer this question!
    15·1 answer
  • __________ is the pressure that propels blood to the tissues.
    15·1 answer
  • Water and minerals are absorbed into the blood in the
    5·1 answer
  • What is likely to happen to the cells of a marine plant if placed in a fresh water environment?
    15·1 answer
  • Why do potato plants no longer need to use glucose from starch in respiration once they have grown above ground?
    13·1 answer
  • What's the opposite of 0.5
    6·1 answer
  • Order the steps of the urine formation process
    9·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!