You are trying to program E. coli bacteria to generate N-acylethanolamide, lipids that have been found to help suppress appetite
. In order to do this, you need to transfer the gene for the enzyme N-acyltransferase from the plant Arabidopsis Thaliana into the E.coli bacteria. The transgene, which codes for ***********, will also need to have a**********
for RNA polymerase and a ********** that would fluoresce to let you know the insertion was successful. You would also need a********** , a small circular piece of bacterial DNA.
Then you would hunt for a *********** that would cut the plasmid once and also cut the transgene out from its host.
The transgene, which codes for N-acylethanolamide, will also need to have a transcription initiation site for RNA polymerase and a selectable marker (reporter gene) that would fluoresce to let you know the insertion was successful. You would also need a plasmid, a small circular piece of bacterial DNA. Then you would hunt for restriction enzymes that would cut the plasmid once and also cut the transgene out from its host.
Explanation:
A transgene can be defiend as a gene (in this case, the N-acylethanolamide gene) which has been transferred by using genetic engineering techniques from one organism to another. The RNA polymerase is an enzyme required to copy a DNA sequence (e.g., a gene) into an RNA sequence, during transcription. RNA polymerases bind to specific DNA sequences called transcription initiation sites on the DNA template, and this binding triggers the initiation of the transcription. A plasmid is a small circular DNA molecule separated from chromosomal DNA which is capable of replicating independently of the chromosomal DNA. Plasmids are widely used in genetic engineering to clone/transfer/manipulate transgenes. During transgenesis, the plasmid is cut with a specific restriction enzyme in order to ligate the transgene of interest into the plasmid. Finally, the green fluorescent protein (GFP) gene is a selectable marker (i.e., a reporter gene) that indicates which cells were successfully modified because its expression can be detected without any substrates.
