Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
If the hydronium concentration increases, the pH decreases, causing the solution to become more acidic. This happens when an acid is introduced. As H+ ions dissociate from the acid and bond with water, they form hydronium ions, thus increasing the hydronium concentration of the solution.
Answer:d.Paleozoic Era
Explanation:
Paleozoic Era is hallmarked by climate, animals & plants.
This Era includes the Ordovician Period, Silurian Period and the Devonian Period.
The Silurian Period is one during this era where aquatic plants and animals evolved.
Answer:
The genotype of each parent plant is 1:2:1
Explanation:
I don't say you have to mark my ans as brainliest but my friend don't forget to thank me if it has really helped you....
The color of beef is determined by myoglobin because these cells are actually responsible for transporting oxygen-rich molecules to the the tissues of the animal. This characteristic gives it the color that it has and is very abundant in large organisms like cattles.
