The Oxidation-Fermentation Test is used to differentiate bacteria built on their capability to oxidize or ferment specific sugars.
Once microbes are inoculated,-One tube is sealed with a layer of sterile mineral oil to promote anaerobic growth and fermentation.-The other tube is left unsealed to allow aerobic growth and oxidation.
Organisms able to ferment the carbohydrate or ferment and oxidize the carbohydrate will turn the sealed and unsealed yellow throughout.
Organisms able only to oxidize the sugar will turn the unsealed yellow medium and leave the sealed medium green or blue.
Fragile fermenters will convert both tubes slightly yellow at the top.
Organisms not able to metabolize the sugar will either produce no color change or will turn the medium blue due to alkaline products from amino acids degradation.
Since Pair #1 showed complete yellowing for sealed and unsealed, these Organisms able to ferment the carbohydrate or ferment and oxidize the carbohydrate. So our interpretation will be that the organism has: Oxidation and fermentation OR fermentation only.
For tubes #2 and #3, the sealed tubes were green throughout suggests that they need oxygen for aerobic growth, and the fact that their unsealed tubes showed light yellowing is evidence for oxidation. Sealed - Green and Unseal - Yellow. Our interpretation for these pairs of tubes would be : Oxidation
Tube 1 can be either Oxidation and fermentation OR fermentation only. So reliability of this needs to be confirmed more with additional testing.
Tubes 2 and 3 are most reliable because they can only be oxidation only and no fermentation.
One of the challenges that scientists face when classifying a new fossil is that any tiny detail is all that separates one species from another. Further exploration is required to identify another set of characteristics that may help in deciding which organism belongs or create a new class.
Explanation:
Sediment discharge was historically approximately 270 million cubic meters/year of suspended load and 130 million cubic meters/year of bedload. This has decreased 80% since 1850 and can be divided into three periods: historical period (pre 1900), pre-dam period (1932-1952), and post dam (1963-1982). Suspended sediment loads declined 43% between the historical and pre-dam and 51% from pre-dam to post-dam periods. The size of sediment also decreased drastically including a 72% decrease in the sand fraction. Most of this is due to dams on the tributaries acting as sediment traps primarily for the coarser sediments. Large-scale land clearing for agriculture contributed to increased sediment loads in the historic period.
The answer to your question is D
The same way we never inject a full bag of anything quickly and intravenously. Your body would not be able to compensate for the amount of the substance. Alcohol or more importantly the Ethanol or Ethyl Alcohol in alcoholic beverages is the active ingredient of such beverages. It is also a drug called a CNS (Central Nervous System) depressant which means it is in the same class category of Opioids (Such as Heroin and Morphine) and Barbiturates (Rophynol). Your body processes alcohol via the liver, as it is also considered a toxin. By consuming a massive amount of alcohol without spacing it out, you will overload your system, not only literally overwhelming your liver and causing Alcohol Poisoning, but you also can go into a state similar to a Heroin overdose.
