Answer: Km = 10μM
Explanation: <u>Michaelis-Menten constant</u> (Km) measures the affinity a enzyme has to its substrate, so it can be known how well an enzyme is suited to the substrate being used. To determine Km another value associated to an eznyme is important: <em>Turnover number (Kcat)</em>, which is the number of time an enzyme site converts substrate into product per unit time.
Enzyme veolcity is calculated as:
![V_{0} = \frac{E_{t}.K_{cat}.[substrate]}{K_{m}+[substrate]}](https://tex.z-dn.net/?f=V_%7B0%7D%20%3D%20%5Cfrac%7BE_%7Bt%7D.K_%7Bcat%7D.%5Bsubstrate%5D%7D%7BK_%7Bm%7D%2B%5Bsubstrate%5D%7D)
where Et is concentration of enzyme catalitic sites and has to have the same unit as velocity of enzyme, so Et = 20nM = 0.02μM;
To calculate Km:
![V_{0}*K_{m} + V_{0}*[substrate] = E_{t}.K_{cat}.[substrate]](https://tex.z-dn.net/?f=V_%7B0%7D%2AK_%7Bm%7D%20%2B%20V_%7B0%7D%2A%5Bsubstrate%5D%20%3D%20E_%7Bt%7D.K_%7Bcat%7D.%5Bsubstrate%5D)
![K_{m} = \frac{E_{t}.K_{cat}.[substrate]-V_{0}*[substrate]}{V_{0}}](https://tex.z-dn.net/?f=K_%7Bm%7D%20%3D%20%5Cfrac%7BE_%7Bt%7D.K_%7Bcat%7D.%5Bsubstrate%5D-V_%7B0%7D%2A%5Bsubstrate%5D%7D%7BV_%7B0%7D%7D)
Km = 10μM
<u>The Michaelis-Menten for the substrate SAD is </u><u>10μM</u><u>.</u>
1. ocean-> seashells -> limestone -> lithosphere
2. ocean -> marine plants -> sediments -> lithosphere
Answer:
its an insulator
Explanation:
they block electricity from passing
Magnesium phosphite maybe
Answer:
1,171 fluid ounces of herbicide is sufficient for 2050 square meter.
Explanation:
If 300 square feet of land is sprayed by the herbicide at the rate of 16 liquid ounce, So 1,171 fluid ounces of herbicide is enough to cover the area of 2050 square meters.
Calculation:
First convert 300 square feet into meter which is equal to 28 square meter.
16 ounces of herbicide = 28 square meter
x ounces of herbicide = 1050 square meter
by cross multiplication we get
28 x = 16 x 1050
x = 32800/ 28
x = 1171 ounces of herbicide.
