Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Answer:
Explained below
Explanation:
When we heat a liquid, what happens is that the molecules of the liquid will absorb heat and thus develop kinetic energy that will make them move faster.
Now, as the liquid begins to boil, bubbles of will be formed inside the liquid and then rises to the surface. Now, when the temperature of the reaches 100°C which is the boiling point of a liquid, the molecules at the top of the liquid begin to change to gaseous state and escape in form of vapour.
Answer:
a. equal to
Explanation:
The <em>osmotic pressure</em> is calculated by the formula:
π = <em>i</em> * M * R * T
Where π is the osmotic pressure, M is the concentration, R is a constant, T is temperature and <em>i</em> is the van't Hoff's factor (the number of ions a compound forms when dissolved in water,<u> for both NaCl and KBr is 2</u>).
Because R is always the same, and <u>Temperature and Concentration are equal between the two solutions</u>, the osmotic pressure of both solutions are also equal.
(80+125+45) / 10 = 250/10 =25
25 meters per minute= 0.41 meters/second
the direction and stopping time is irrelevant to the problem.
Camels lose less water through their urine and feces than many other mammals. Their kidneys concentrate water heavily, leading to salty urine. The intestines also reabsorb water from intestinal material as it is digested, so dry feces are produced.
