In step 2: Denaturation of the double-strand occurs.
In step 3: Annealing of the primer to the single strands.
In step 4: Extension or elongation takes place in this step.
Explanation:
In the PCR program an enzyme Taq Polymerase is used because it can withstand high temperature without altering its functions.
PCR is required for the amplification of DNA into multiple copies for experimental purpose. The artificial environment is created to form new DNA molecules from the sample in question.
The first step in replication is the opening of the double helix which is done by temperature treatment in PCR. The temperature would be 90 degrees for some 30 sec to two minutes.
The next step of primer annealing would be done at 52 degrees, this is the primer melting temperature.
The elongation of the DNA strand to be synthesized will take place at 72 degrees as Taq Polymerase can withstand that temperature.
Nearly one million copies of DNA will be made after 30 cycles of PCR.
PCR products can be stored at 4 degrees for some two months.
I don’t know what you’re asking sir.
Answer: Oil resources
Explanation:hope this helps and happy early hallowen :D
A. a six carbon molecule of glucose is converted to 2 sets of pyruvates, or pyruvaic acids. Glycolysis - "glyco"- sugar "lysis"-breaking down. So glycolysis means splitting of glucose.
<span>In one sample, the potatoes that have more sugar will also have more water that will diffuse into them. Potatoes are hypertonic in relation to pure water,simply put, they will gain extra water weight as well as displace more water than potatoes that are lower in sugar. In a separate sample, potatoes that have a higher concentration of sugar will also lose far less water due to their lack of hypotonic nature. Those with lower sugar will easily loose more water.</span>
