Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
Answer:
<em><u>FACT</u></em>
Explanation:
<h3>Cells as Building Blocks</h3>
<h3>A living thing, whether made of one cell (like bacteria) or many cells (like a human), is called an organism. Thus, cells are the basic building blocks of all organisms</h3>
<span>Vacuoles are organelles present in plant cells, some animal, and some prokaryotic cells. There is a difference in size between plant and animal cells. Plant cells usually have one large vacuole while animal cells have a number of small vacuoles. If the term "small vacuoles" is used for vacuoles in animal cells, then they are involved in overcoming the foreign particles, storage of waste and during the exocytosis, and invagination of the cell membrane during engulfing the bacteria.</span>
<span>It can eaither be PP or Pp.</span>
Answer:
In this photo, one can observe the big mountains, the clear blue sky, the sand which covers the ground, the path down the middle and the variety of dry, green plants.
Explanation:
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