Answer:
D) Because sticky ends can be temporarily held together by hydrogen bonding between the two strands.
Explanation:
Restriction enzymes cut the DNA at specific restriction sites and by the mechanism of action they can form two types of ends:
- sticky ends-single-stranded overhangs are formed
- blunt ends-without overhangs.
The main advantage of sticky ends (their overhangs) is that they can complementary bind to another overhand formed by the same restriction enzyme. So, for example in cloning, if the DNA of interest and plasmid vector are cut with the same restriction enzyme, that forms sticky ends, fragment of DNA will fit into a bacterial plasmid in one direction.
On the other hand, blunt ends can be inserted into vector in both directions: head-to-tail or tail-to-head.
In DNA replication, the new strand of dna that is synthesized in one continuous piece is called the leading strand
Answer:
Chemical bonds include covalent, polar covalent, and ionic bonds. Atoms with relatively similar electronegativities share electrons between them and are connected by covalent bonds. Atoms with large differences in electronegativity transfer electrons to form ions. The ions then are attracted to each other.
Two of the strongest forms of chemical bond are the ionic and the covalent bonds. Chemical bonds form between two atoms, each with its own electron environment.
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