Adaptation is a phenomenon that is about adjusting certain features to environmental conditions. Ie. birds are adapted to flight as they possess wings, very efficient lungs etc. or tropical plants are adapted to high temperature and humidity as they modified their photosynthesis process. but if some creature is adapted to the specific habitat or conditions, it is hard for it to manage in another habitat/different conditions. ie. birds are fast in the air but on the ground they are quite weak and prone to predators' attacks.
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
It is true that an organism that is asymmetrical can not be divided into identical or mirror images.
Answer:
<u>All the substrates are bound to the enzyme</u>, thats why the reaction rate stopped increasing.
Explanation:
Actually an enzyme works by binding a substrate molecule to its active site, producing a product , resulting in an increase in the rate of reaction.
Enzyme + substrate = Enzyme + product
Whenever enzymes are added , reaction rate increases as the substrates are being bound to the enzyme's active sites but then occurs a stage where further addition of enzyme cannot increase the reaction rate because all the substrates have bound to the enzyme and <u>there are no substrates left.</u> Hence the reaction rate stopped increasing.
