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The standard addition equation is as followsI_(S+X) (V/V_O )=I_X+I_X/[X]_i [S]_4 (V_S/V_0 ) Here, [X]_i is the initial concentration of analyte, [S]_i is the initial concentration of standard, I_X is signal for analyte, I_(S+X) is signal for standard and analyte, V_0 is the initial volume, V_S is the added standard volume, and V is the total volume.Added volume of standard V_S is-23.3 mL. Initial volume of the sample V_0 is 10.00 mL. Initial concentration of standard ([S]_i) is 0.156 ng/mL.[X]_i= -[S]_i (V_S/V_0 )〖[X]〗_(i )= -(0.156 ng/mL)((-23.3 mL)/(10.00 mL))=0.363 ng/mL
Concentration of U(III) in ground sample is 0.363 ng/mL
The combination sounds like it'd be a powerful disinfectant, but the two should never be mixed. "Together, they produce chlorine gas, which even at low levels, can cause coughing, breathing problems, and burning, watery eyes," says Forte.
Answer:
50 mL
Explanation:
We can solve this problem by using Boyle's Law, which states that:
"For a fixed mass of an ideal gas kept at constant temperature, the pressure of the gas is inversely proportional to its volume"
Mathematically:
where
p is the pressure of the gas
V is the volume of the gas
The equation can be rewritten as
where in this problem:
is the initial pressure of the gas in the coke
is the initial volume
is the final pressure
Solving for V2, we find the final volume:
Answer:
High concentration of glutathione should be included in the elution buffer for the given experiment to remove protein from the column.
Explanation:
Affinity chromatography is one of important biochemical technique of chromatography which depends on the affinity of ligand for the receptor.Here the ligand is a protein mixture which act as mobile phase and the receptor is present in the wall of chromatography column act as stationary phase.When the protein mixture is applied on the top of the column the substances present within the protein that have high affinity for the receptor present in the walls of chromatography column binds to the later but rest of the protein pass away through the column.High concentration of ligand is used within an buffer solution to remove the desired protein from the column.
From this point of view it can be stated that in the given question high concentration of glutathione should be used to remove the desired protein from the column.
