Answer:
(a) 133.33nm
(b) 600nm
(c) 7,600nm
Explanation:
The concentration of Y can be determined by using the formula:
![Y = \dfrac{[L]}{k_d+[L]}](https://tex.z-dn.net/?f=Y%20%3D%20%5Cdfrac%7B%5BL%5D%7D%7Bk_d%2B%5BL%5D%7D)
where;
[L] = concentration of the binding ligand.
kd = 400 nm
Thus:
When Y = 0.25; we get :
![0.25 = \dfrac{[L]}{400+[L]}](https://tex.z-dn.net/?f=0.25%20%3D%20%5Cdfrac%7B%5BL%5D%7D%7B400%2B%5BL%5D%7D)
0.25 (400 + [L]) = [L]
100 + 0.25[L] = [L]
100 = [L] - 0.25 [L]
100 = 0.75 [L]
[L] = 100/0.75
[L] = 133.33 nm
At, Y = 0.6
![0.6 = \dfrac{[L]}{400+[L]}](https://tex.z-dn.net/?f=0.6%20%3D%20%5Cdfrac%7B%5BL%5D%7D%7B400%2B%5BL%5D%7D)
0.6 (400 + [L]) = [L]
240 + 0.6[L] = [L]
240 = [L] - 0.6 [L]
240 = 0.4 [L]
[L] = 240/0.4
[L] = 600 nm
At, Y = 0.95
![0.95 = \dfrac{[L]}{400+[L]}](https://tex.z-dn.net/?f=0.95%20%3D%20%5Cdfrac%7B%5BL%5D%7D%7B400%2B%5BL%5D%7D)
0.95 (400 + [L]) = [L]
380 + 0.95[L] = [L]
380 = [L] - 0.95 [L]
380 = 0.05 [L]
[L] = 380/0.05
[L] = 7600 nm
Answer:
Hexane should be used first.
Explanation:
Chromatography is a method of separating the constituents of a mixture by taking advantage of their different rates of movement in a solvent over an adsorbent medium. Chromatography is a means of separation and analysis that utilises fractional separation. It is based on the principle that if a fluid containing a number of substances is allowed to pass though an adsorbent medium, the different substances in the fluid may travel at different rates and be separated.
The rate of movement depends on the relative affinities of the constituents for the solvent and adsorbent medium. i.e solutes which are weakly adsorbed by the adsorbent medium are easily redissolved by the ascending solvent and quickly travel up the adsorbent medium. In addition to that , solutes which are very soluble in the solvent move up at a faster rate than those which are not soluble.
In column Chromatography;
A non-polar solvent should be initiated and applied first. This is because , in a column chromatography, a non-polar compound will be removed at first then later polar compound.
Assuming a polar compound is used first, the polar compounds will be removed alongside with all the non-polar compounds.
From the two Compounds given;
We know that :
Hexane is a non-polar compound and Methylene chloride is a polar compound. As such, Hexane should be used first.
The volume at STP : 9.856 L
<h3>Further explanation</h3>
Given
Mass of ethane : 13.21 g
Required
The volume at STP
Solution
Conditions at T 0 ° C and P 1 atm are stated by STP (Standard Temperature and Pressure). At STP, Vm is 22.4 liters / mol.
mol ethane(C2H6) :
= mass : molar mass
= 13.21 g : 30 g/mol
= 0.44
Volume at STP :
= 0.44 x 22.4 L
= 9.856 L
Your question has been heard loud and clear.
Both scientific laws and scientific theories are not based on hypothesis.
Because , scientific laws are proven and so they are real not hypothetical.
Whereas scientifc theories can be hypothetical.
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