I would say,
Aa.
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Skipper flies
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Hi
Below are five major steps of DNA or gene cloning:
1: You chose the gene or specific piece of DNA you need to clone and cut the gene with restriction enzymes from the source organism.
2: You need to choose a vector for the process of cloning and you will cut the vector with same restriction enzymes through which you have cut your target DNA sequence to be inserted into the vector.
3: You place the target gene into the vector and join or seal the gene with vector by using an enzyme called DNA ligase.
4: You introduce the vector with your target gene into a suitable host organism such as yeast or bacteria through the process of Transformation. In this process host organism takes up the vector containing your target gene and starts replicating the target DNA along with their own DNA and thus creating millions of copies of target gene .
5: In the last step, the DNA or target gene is isolated from host organism and purified and is ready to be used since its quantity has been enormously increased through the process of cloning.
The cloning is also called as recombinant DNA technology and is the main process that is being used in the production of insulin for diabetes patients. You can see below image for better understanding.
Hope it help!
Answer:
Three different kinds of muscles are -:
- <u>SKELETAL MUSCELES </u>
- <u>CARDIAC MUSCLES </u>
- <u>SMOOTH MUSCLES</u>
Explanation:
- <u>SKELETAL MUSCLES -: </u>There are long, cylindrical, and striated skeletal muscle cells. They are multi-nucleated, which means they have more than one nucleus. This is because from the fusion of embryonic myoblasts, they are created. Each nucleus controls the sarcoplasm's metabolic demands around it. There are high energy requirements for skeletal muscle cells, because they contain several mitochondria in order to generate adequate ATP. <u>Examples of skeletal muscles: arms and legs- </u>T<u>he muscles that belong to the arms and legs feature in pairs. Abdomen and Back- These muscles are connected to the various sets of skeletal muscles that run across the torso.</u>
- <u>CARDIAC MUSCLES -</u>: Cardiomyocytes have a short and narrow outline and are fairly rectangular. They are about 0.02 mm wide and 0.1 mm (millimetres) long, respectively. There are many sarcosomes in cardiomyocytes, which provide the required energy for contraction. Cardiomyocytes usually contain a single nucleus, unlike skeletal muscle cells. Cardiomyocytes, although they contain more sarcosomes, normally contain the same cell organelles as skeletal muscle cells.<u> example - cardiac muscle is present in heart. </u>
- <u>SMOOTH MUSCLES -:</u> Smooth muscle cells have a single central nucleus and are spindle-shaped. They range in length from 10 to 600 μm (micrometers), and are the tiniest type of muscle cell. In the expansion of organs like the kidneys , lungs, and vagina, they are elastic and therefore essential. As in cardiac and skeletal muscle, the myofibrils of smooth muscle cells are not aligned, meaning they are not striated, hence the term smooth. <u>example of smooth muscles -: Walls of blood vessels , Walls of stomach ,
Ureters , Intestines , In the aorta (tunica media layer), Iris of the eye. ,Prostate and Gastrointestinal Tract.</u>
Cell Differentiation
Giant sequoias can grow more than 80 meters tall! molecules they need for growth using atoms from the water and air around them. ... Plants take in molecules from water and air around them to make different molecules that can be used for energy and growth.
meristematic tissues
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