Answer:
gDNA = "genomic DNA" and cDNA = "complementary DNA." cDNA is classically associated with being reverse transcribed either from all extracted RNA from a tissue or cell (total RNA) including (in eukaryotes) pre-mRNA, ribosomal RNA, tRNA, snoRNA, miRNA and mRNA, etc.) while cDNA obtained only from reverse transcription of the mRNA (expressed eukaryotic cytosolic mRNA) fraction (e.g., by poly[dT]n and random priming) is complementary DNA (cDNA) made from what is called the "transcriptome." Eukaryotes have introns and exons in the gDNA, while prokaryotes do not. So eukaryotic cDNA reverse transcribed from mRNA lacks introns. Prokaryotic-derived cDNA is always complementary to prokaryotic RNA and gDNA (so is always necessary to have a good DNase treatment prior to gene expression analysis by e.g., qPCR for prokaryotic transcriptome work)...
At least 12 million years ago?
Answer: Many pathogenic fungi are parasitic in humans and are known to cause diseases of humans and other animals. In humans, parasitic fungi most commonly enter the body through a wound in the epidermis (skin). Such wounds may be insect punctures or accidentally inflicted scratches, cuts, or bruises. One example of a fungus that causes disease in humans is Claviceps purpurea, the cause of ergotism (also known as St. Anthony’s fire), a disease that was prevalent in northern Europe in the Middle Ages, particularly in regions of high rye-bread consumption. The wind carries the fungal spores of ergot to the flowers of the rye, where the spores germinate, infect and destroy the ovaries of the plant, and replace them with masses of microscopic threads cemented together into a hard fungal structure shaped like a rye kernel but considerably larger and darker. This structure, called an ergot, contains a number of poisonous organic compounds called alkaloids. A mature head of rye may carry several ergots in addition to noninfected kernels. When the grain is harvested, much of the ergot falls to the ground, but some remains on the plants and is mixed with the grain. Although modern grain-cleaning and milling methods have practically eliminated the disease, the contaminated flour may end up in bread and other food products if the ergot is not removed before milling. In addition, the ergot that falls to the ground may be consumed by cattle turned out to graze in rye fields after harvest. Cattle that consume enough ergot may suffer abortion of fetuses or death. In the spring, when the rye is in bloom, the ergot remaining on the ground produces tiny, black, mushroom-shaped bodies that expel large numbers of spores, thus starting a new series of infections.
Answer:
* Glucose may cause an increase in bacterial growth - HYPOTHESIS
* The bacterial colonies grown without glucose don’t have capsules around their cells - OBSERVATION
* Bacterial growth in the glucose environment increased rapidly and then stopped after some time - OBSERVATION
* Increased glucose may lead to capsule formation in bacteria - HYPOTHESIS
Explanation:
In an experiment, a HYPOTHESIS is a predictive and testable statement that aims to answer a scientific question while an observation is made based on the results obtained.
According to this question, Annie wants to study the effect of glucose on bacterial colonies. She conducted the experiment by growing two colonies of bacteria - one with glucose present in the environment and one in an environment without glucose. Based on this experiment, the following statements are categorized as:
- Glucose may cause an increase in bacterial growth is a HYPOTHESIS
- The bacterial colonies grown without glucose don’t have capsules around their cells is an OBSERVATION
- Bacterial growth in the glucose environment increased rapidly and then stopped after some time is an OBSERVATION
- Increased glucose may lead to capsule formation in bacteria is a HYPOTHESIS
