<span>The answer is the first option. Enzyme and substrate bind using a lock-and-key mechanism. Enzymes act on a specific substrate and a substrate needs a specific enzyme, this is what is called a lock-and-key mechanism. Enzymes and substtates are like a key and a lock, one is for each other.</span>
<span>all of the above can be saturated molecules </span>
Due to hydrogen bonding there is a formation of cage like structure called lattice in ice due to which <span> density of ice is less than that of water. Moreover, it is a known fact that density of water is maximum at 4°C.</span>