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erik [133]
2 years ago
13

can someone help me with this immediately!!!! pls don’t guess or leave links!! correct answer gets brainiest

Biology
1 answer:
sdas [7]2 years ago
5 0
The answer is the heart
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6. (3 pts) After transformation, 40 colonies were present on the plate. What is the transformation efficiency of the competent c
natali 33 [55]

Answer:

Given:

Number of colonies on plate = 40

Amount of DNA used for transformation = 100ng

Volume of competent cells used = 100ul

Total volume = 1ml (1000ul)

Volume plated for growth = 200ul

The amount of cells transformed per 1 µg of DNA is called the transformation efficiency

(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA

Amount of DNA used for plating = 100ng * (200/1000)

                                                      = 100ng * (1/5)

                                                      = 100/5 = 20ng

Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng

               (2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA

Transformation efficiency = 2000 Transformants / ug DNA

                                          = 2 x 10³ Transformants / ug DNA

Explanation:

Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.

6 0
2 years ago
How does global warming effect climate change?​
anzhelika [568]

Answer:

world go warm climate go hot

5 0
2 years ago
Need right now will mark brainliest if right
mezya [45]

Answer:

The third diagram shows Refraction of waves.

Explanation:

Refraction is a phenomenon, which occurs in a wave when it travels from one medium to another medium, and during Refraction there is slight or greater change in direction of the wave.

hence, the wave will pass through the surface and displaces from its actual path.

that's why the most appropriate diagram showing Refraction is third one ( c ) .

3 0
3 years ago
The resonators are the mouth, nose, and lips. true or false?
galina1969 [7]
The answer is false. Hope this helps
3 0
3 years ago
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Hello! I have a question and I ask if you can make it a bit detailed. I am struggling to understand the Calvin Cycle in the proc
igomit [66]

Absolutely!  The Calvin Cycle is a process that takes place within the stroma of chloroplasts during photosynthesis.  This cycle is a light-independent reaction because it does not need sunlight to fully develop.  The process is, however, dependent on ATP and NADHP from those light reactions.

If you want a really good study guide for the Calvin Cycle, just follow this link; it helps A LOT!

https://www.khanacademy.org/science/biology/photosynthesis-in-plants/the-calvin-cycle-reactions/a/calvin-cycle

Hope this helps!! :)

3 0
3 years ago
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