Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
world go warm climate go hot
Answer:
The third diagram shows Refraction of waves.
Explanation:
Refraction is a phenomenon, which occurs in a wave when it travels from one medium to another medium, and during Refraction there is slight or greater change in direction of the wave.
hence, the wave will pass through the surface and displaces from its actual path.
that's why the most appropriate diagram showing Refraction is third one ( c ) .
The answer is false. Hope this helps
Absolutely! The Calvin Cycle is a process that takes place within the stroma of chloroplasts during photosynthesis. This cycle is a light-independent reaction because it does not need sunlight to fully develop. The process is, however, dependent on ATP and NADHP from those light reactions.
If you want a really good study guide for the Calvin Cycle, just follow this link; it helps A LOT!
https://www.khanacademy.org/science/biology/photosynthesis-in-plants/the-calvin-cycle-reactions/a/calvin-cycle
Hope this helps!! :)
