The layers above would be not as old and the layers below would be the older layers.
D. an RNA molecule since the RNA molecule contains information for making proteins
The first major difference between prokaryote and eukaryote gene expression is the location. Due to lack of compartments (nucleus) prokaryotic gene expression (both transcription and translation) occurs within the cytoplasm (genetic material is located within the cytoplasm). So, transcription and translation in prokaryote occurs simultaneously. Gene expression in eukaryote occurs in both the nucleus (transcription) and cytoplasm (translation).
The steps of regulation of gene expression are also dissimilar, in prokaryotic cell, transcription is mostly the main point of regulation (eukaryote have transcription, post-transcription, translation, and post-translation regulation together with epigenetic regulation).
The answer is dusk and dawn.
Crepuscular animals are active during twilight (latin crepusculum). Twilight is a period after dawn and before dusk, so crepuscular animals want to avoid dusk and dawn. Animals' activity in different periods during the day depends on temperature or other biotic factors. It also depends on predators' activities or local competition.
You didn't really provide the required table. But as a general hint, you should look at the differences between the two groups and then you can see what were the differences in amount of weight gained, amount of body fat, as well as the composition of the microbial community.