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Their skeletal structure and possibly how they look but usually not. You can also look at the way they live and the biomes they live in.
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considered what? the question was not finished
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Answer: The answer is, use of fertilizers (manure) in farming this is on study island 
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A. behavioral isolation is when the plant or animal acts a particular way to attract their counterparts, like mating rituals.
geographic isolation is the natural barrier preventing similar species from interacting. for example, Galapagos Islands are the only home of flightless cormorants because the islands are so far from any other land for them to invade.
Temporal isolation is when two of the same or similar species cannot reproduce as a result of mismatched sexual maturity.
B. the isolation of two frog species by mating call is behavioral isolation.