Answer:
Each mutant would be mated to wild type and to every other mutant to create diploid strains. The diploids would be assayed for growth at permissive and restrictive temperature. Diploids formed by mating a mutant to a wild type that can grow at restrictive temperatures identify the mutation as recessive. Only recessive mutations can be studied using complementation analysis. Diploids formed by mating two recessive mutants identify mutations in the same gene if the diploid cannot grow at restrictive temperature (non-complementation), and they identify mutations in different genes if the diploids can grow at restrictive temperature (complementation).
Explanation:
Recessive mutations are those whose phenotypic effects are only visible in homo-zygous individuals. Moreover, a complementation test is a genetic technique used to determine if two different mutations associated with a phenotype colocalize in the same <em>locus</em> (i.e., they are alleles of the same gene) or affect two different <em>loci</em>. In diploid (2n) organisms, this test is performed by crossing two homo-zygous recessive mutants and then observing whether offspring have the wild-type phenotype. When two different recessive mutations localize in different <em>loci</em>, they can be considered as 'complementary' since the heterozygote condition may rescue the function lost in homo-zygous recessive mutants. In consequence, when two recessive mutations are combined in the same genetic background (i.e., in the same individual) and they produce the same phenotype, it is possible to determine that both mutations are alleles of the same gene/<em>locus</em>.
Biotic factors include animals, plants, fungi, bacteria, and protists. Some examples of abiotic factors are water, soil, air, sunlight, temperature, and minerals.
Answer:

Explanation:
Given -
Total Population - 
Individuals with homozygous HbA/hbA genotype - 
Individuals with homozygous hbs/hbs genotype -
Individuals with heterozygous hba/hbs genotype - 
Let us assume the given population is in Hardy Weinberg' equilibrium
The frequency of individuals in the given population with homozygous HbA/hba genotype is equal to number of individuals with homozygous HbA/hba genotype divided by total population.

The frequency of hba allele is equal to

Answer:
Answer : Depolarized
Explanation:
Sodium and Potassium ions tend to move inside of the axin when the membrane is depolarized.
A change in polarity occurs in the axon membrane when a nerve impulse travels across it. At the beginning of an action potential or nerve impulse , the ions of sodium tend to move inside of axon causing depolarization. As a result potassium ions tend to move outside of the axon causing re polarization. Hence, this effect causes the nerve impulse to travel in one direction only inside the axon.