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Since the 2 strands of DNA are antiparallel to on another, DNA must be replicated both continuously and dis-continuously. The side of DNA that is replicated dis-continuously is done so in small parts called <u>okazaki fragments</u>.
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OKAZAKI FRAGMENTS - Okazaki fragments are short DNA nucleotide sequences (roughly 150 to 200 base pairs in eukaryotes) that are synthesized in a non-linear fashion and then linked together by the enzyme DNA ligase to form the lagging strand during DNA replication. They shape short double-stranded DNA sections when combined with the lagging template strand.
The primosome initiates Okazaki fragments by generating a new RNA primer. To restart DNA synthesis, the DNA clamp loader releases the lagging strand from the sliding clamp and reattaches the clamp to the new RNA primer. DNA polymerase III will then synthesize the segment of DNA.
Reiji Okazaki, Tsuneko Okazaki, and their colleagues first found Okazaki fragments in 1968 while researching bacteriophage DNA replication in E. coli.
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