The answer to that question is no
The human gene mutation database (HGMD) is a collection of published germ-line mutations in nuclear genes that underlie , or are closely associated with human inherited disease.
For identifying the pathogenic mutation among thousands to millions of genomic variant is a major challenges and prioritization strategies are required. Current strategies for Mendelian disease gene identification by exome re-sequencing.
Next generation sequencing (NGS) has been used to identify new pathogenic mutations and genes causing rare genetic diseases. The analyses of NGS data is not trivial and required a technically and biologically rigorous pipeline that address the data quality , accurate variant filtration to minimize mistakes.
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Answer: I believe it is 3.) Denature
In serial or tandem staging<span> schemes, the first </span>stage<span> is at the bottom and is usually the largest, the </span>second stage<span> and subsequent upper </span>stages<span> are above it, usually decreasing in size. In parallel </span>staging<span> schemes solid or liquid </span>rocket<span> boosters are used to assist with lift-off.</span>
