Monoclonal antibodies are made by the fusion of cells of the immune system with B lymphocytes and myeloma cells.
<h3>What is Hybridoma technology?</h3>
- It is one of the best technologies that is used to produce the monoclonal antibody.
- In this hybridoma technology the B lymphocytes that are antibody producing are isolated from a source after the immunization with a specific antigen and then are fused with myeloma cell line to form hybrid cells that are also called as hybridoma cell lines.
- The hybridoma cell lines are then cultured in the laboratory with specific antigen and then the monoclonal antibodies are produced.
- This can be done in in-vivo or in-vitro condition.
- This method is preferred over all because the production of antibodies by this method is good as the antibodies produced are of high purity and highly sensitive and specific.
- These cell lines can also be preserved for a long time.
- Hybridoma technology has resulted in production of different varieties of monoclonal antibodies with specificity with specific antigens as the monoclonal antibodies are produced by single parental B cells.
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True. Some bacterial cells are resistant to a variety of antimicrobials because they actively pump the drugs out of the cell.
A significant resistance mechanism in Gram-negative bacteria is drug efflux. It expel solutes from the cell. Antimicrobials and metabolites are just a few of the hazardous compounds that Efflux pumps help bacteria remove from their interior environments so they can regulate it.
The main efflux systems in Gram-negative bacteria are members of the RND superfamily and typically consist of an outer membrane protein channel, a periplasmic protein, and a cytoplasmic membrane pump. The most common example is MFS (such as Bmr and Blt in Bacillus subtilis) and the ABC transporters.
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As a greenhouse gas that absorbs heat, CO2 raises the temperature of the atmosphere. We refer to this as global warming. The glaciers will melt more quickly as the world's temperature rises, raising sea levels and bringing on disaster. More power is consumed for air conditioning as the temperature rises, which further raises the air's CO2 level.
Since coal is the primary fuel utilized in the creation of power. This creates a vicious cycle.
