This is the order;
<span>1.
</span>Extract mRNA from cells
<span>2.
</span>Reverse transcribe mRNA to cDNA
<span>3.
</span>Label cDNA with a chemiluminescent molecule
<span>4.
</span>Add cDNA to microarray and incubate
<span>5.
</span>Wash away unbound cDNA
<span>6.
</span>Visualize microarray and analyze the data
When you start the PCR amplification reaction, you have a template which has sequences where the primers will anneal. The forward primer anneals in the sense strand and the reverse one in the complementary sequence (see attached figure).
After the first PCR cycle, two types of fragments are obtained. First those which come from tha 5'-3' sequence, that are 3'-5' and will anneal the reverse primer in the following cycle. This fragments have the correct 3' end, but the 5' includes part of the template that doesn't correspond to our target (see attached figure)
Second, those fragments that come from the 3'-5' sequence, that are 5'-3' and will anneal the forward primer. This fragments also have the correct 3' end, but the 5' includes part of the template that doesn't correspond to our target (see attached figure).
Now, during the second cycle, are produced the first fragments that have the precise length of the sequence that we want to amplify (see attached figure).
So, it's not until the third cycle that the reaction starts amplifying exactly the sequence of desired length.
The question is incomplete as it lacks the group of choices required to answer. However, the correct answer on the basis of general information and knowledge is as follows:
Answer:
The cell theory expresses that: all living things are made out of at least one cell; the cell is the essential unit of life; new cells emerge from existing cells.
- Robert Hooke, an English researcher, watched little compartments in a slight cut of oak bark; he called them cells.
- Anton van Leeuwenhoek, a Dutch researcher, utilized a magnifying lens to watch living lake living beings.
- Researchers found these cells in all creatures that they watched. it was also evident before forming cell theory that cell arises from cells.
Answer:
the carnivore population(s) would increase because their amount of food would increase also
Explanation:
Answer:
The carbon cycle with 4 boxes connected by arrows. Box 1: carbon dioxide in the atmo… ... 4 boxes connected by arrows. Box 1: carbon dioxide in the atmosphere. Box 2: X. Box 3: consumers eat producers. Box 4: Y. The arrow that follows box 4 connects to box 1. Which labels best complete the flow chart?Explanation:
