This affected slaves in a negative way because since the fugitive slaves would be returned to their owner, the slaves would later suffer punishment, beatings, and even death due to them escaping their master. It was a terrible agreement that no state could stop a fugitive slave from being returned to his/her owner.
Endospore staining is used to stain the spore which reside inside the microorganisms, and unlike the exospores, are not present outside the microbial cells. The endospore stain penetrates the microbial cells, and then stain the endospore.
Physical biological and chemical
<span>Mesozoic Era is the anwser</span>