The expressed gene must be incorporated into the bacterial chromosome, but its promoter region should be replaced with a bacterial promoter.
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What about bacterial chromosome?</h3>
- The term "nucleoid" refers to a bacterial chromosome.
- It has genetic material inside and is a strange shape. It may also be known as a genophore.
- Both transcriptional activators and repressors control gene expression in eukaryotic cells.
- Eukaryotic repressors bind to specific DNA sequences and prevent transcription, just like their prokaryotic counterparts.
- Since transcription and translation are physically distinct processes, eukaryotic gene expression is more complex than prokaryotic gene expression.
- Eukaryotic cells, as opposed to prokaryotic cells, have a wide range of levels at which they can control gene expression.
- While transcription and translation can both happen at the same time in bacteria, in eukaryotes, transcription takes place in the nucleus and translation happens in the cytoplasm.
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Disclaimer: The question was given incomplete on the portal. Here is the complete question.
Question: It is often desirable to express eukaryotic genes in bacteria, which can make a protein of interest quickly and cheaply. What is TRUE about this kind of genetic transformation?
a. The expressed gene and the genes for all of its regulatory elements must be incorporated into the bacterial chromosome.
b. Only polycistronic genes are capable of being expressed in a bacteria.
c. The expressed gene and the gene for RNA Polymerase II must both be incorporated into the bacterial genome.
d. Only the transcribed region of the gene should be incorporated into the bacterial genome. No promoter is necessary to transcribe a transformed gene.
e. The expressed gene must be incorporated into the bacterial chromosome, but its promoter region should be replaced with a bacterial promoter.
<span>Cocci also known as a spherical shape
Bacilli also known as a rod-shape
Spirochaete also knows as a spiral-shape</span>
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Having one theory is setting a limit on your potential and the potential of the overall theory and subject you are working on. Having multiple theories gives you a broader idea and perspective of what is going on, and even though one may slightly contradict the other, it is okay because that is what theories are for.
To the point, scientists usually have more than one theory so that they can gain a broader perspective on the matter and maybe even increase the chance of being correct on the matter.
Hope this was helpful.
Cheers,
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